Test Principle The principle works as such that biotin and FITC and – if applicable – digoxigenin and FITC labeled compounds are introduced into the test mixture. These compounds form complexes, which are finally detected by the universal lateral flow strips, Milenia HybriDetect.

Hybridoma Technology Specific mouse monoclonal antibodies can be produced by the Hybridoma Technology. This method results in immortal hybrid cells formed by B-lymphocytes and myeloma cells. After the main steps (immunization of the host animal, B-cell and myeloma cell fusion) the experimenter

The amplification of nucleic acids has become an important tool for various areas such as medical diagnostics, food safety and research over the past decades. A quick and easy way to get a visible result of the amplification is to evaluate the amplification product using the Milenia HybriDetect

HybriDetect helps diagnose viral infections and post-transplantation Monitoring with SHERLOCK Kaminski et al. developed a fast and easy test to detect causes of graft loss in blood and urine samples from post-transplant patients after kidney transplantation.

SHERLOCK and DETECTR are combinations of isothermal amplification and sensitive CRISPR/Cas-based amplicon recognition. The compatibility with the rapid, simple and equipment-free HybriDetect lateral flow platform is an essential feature of these methods.

What is CRISPR/Cas? The CRISPR / Cas system acts as an immune system equivalent that is anchored in the genome of bacteria and archaea. The underlying mechanism protects the organism from phage infections by sequence specific destruction of “unknown” nucleic acids.